BB and Inflammation
Itinerary - View Abstract: "Abstract View
INHIBITORY EFFECTS OF BLUEBERRY POLYPHENOLS ON THE PRODUCTION OF PROINFLAMMATORY MEDIATORS IN ACTIVATED MICROGLIAL CELLS
F.C.Lau*; D.F.Bielinski; J.A.Joseph
Neuroscience Laboratory, USDA, HNRCA at Tufts, Boston, MA, USA
Microglia are the major protectors of the immune system in the central nervous system (CNS). Acutely activated microglia, by brain injury or infection, produce cellular and metabolic alterations that are beneficial to the microenvironment of the CNS. However, pathological activation of microglia has been demonstrated to contribute to the progressive damage in neurodegenerative diseases such as stroke, Alzheimers disease (AD), multiple sclerosis (MS) and human immunodeficiency virus (HIV)-associated dementia. This occurs via the sustained up-regulation of microglial genes to inflammation and the generation of various proinflammatory mediators including nitric oxide (NO), tumor necrosis factor-alpha (TNF-), and interleukin-1 beta (IL-1). Blueberries are rich in anthocyanins, which are among the plant polyphenols that have potent antioxidant and anti-inflammatory activities. The current study showed that treatment with blueberry (BB) extracts significantly and dose-dependently reduced the lipopolysaccharide (LPS)-induced NO production in conditioned media from BV2 murine microglial cells. Reactive oxygen species (ROS) release was also reduced in BB-treated LPS-activated BV2 cells. In addition, BB extracts significantly attenuated the protein expression of the inducible NO synthase (iNOS), cyclo-oxygenases 2 (COX-2), and the pre-processed form of IL-1 in the LPS-activated BV2 cells. Furthermore, the secretion of the inflammatory cytokines IL-1 and TNF- into the conditioned media from the LPS-activated BV2 cells was inhibited by BB treatment. The results from this study suggest that BB polyphenols attenuate inflammatory responses of the brain microglial cells a"
INHIBITORY EFFECTS OF BLUEBERRY POLYPHENOLS ON THE PRODUCTION OF PROINFLAMMATORY MEDIATORS IN ACTIVATED MICROGLIAL CELLS
F.C.Lau*; D.F.Bielinski; J.A.Joseph
Neuroscience Laboratory, USDA, HNRCA at Tufts, Boston, MA, USA
Microglia are the major protectors of the immune system in the central nervous system (CNS). Acutely activated microglia, by brain injury or infection, produce cellular and metabolic alterations that are beneficial to the microenvironment of the CNS. However, pathological activation of microglia has been demonstrated to contribute to the progressive damage in neurodegenerative diseases such as stroke, Alzheimers disease (AD), multiple sclerosis (MS) and human immunodeficiency virus (HIV)-associated dementia. This occurs via the sustained up-regulation of microglial genes to inflammation and the generation of various proinflammatory mediators including nitric oxide (NO), tumor necrosis factor-alpha (TNF-), and interleukin-1 beta (IL-1). Blueberries are rich in anthocyanins, which are among the plant polyphenols that have potent antioxidant and anti-inflammatory activities. The current study showed that treatment with blueberry (BB) extracts significantly and dose-dependently reduced the lipopolysaccharide (LPS)-induced NO production in conditioned media from BV2 murine microglial cells. Reactive oxygen species (ROS) release was also reduced in BB-treated LPS-activated BV2 cells. In addition, BB extracts significantly attenuated the protein expression of the inducible NO synthase (iNOS), cyclo-oxygenases 2 (COX-2), and the pre-processed form of IL-1 in the LPS-activated BV2 cells. Furthermore, the secretion of the inflammatory cytokines IL-1 and TNF- into the conditioned media from the LPS-activated BV2 cells was inhibited by BB treatment. The results from this study suggest that BB polyphenols attenuate inflammatory responses of the brain microglial cells a"
posted by Tim | 1:06 PM
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